10 minutes maximum! Can you do it in 5?

1. Many crop plants have been genetically engineered. Which of the following is an example of genetic engineering?

  • A. Plants being grown hydroponically
  • B. A gene for herbicide resistance being transferred into the crop plant from another species.
  • C. Crop plants being grown without the use of chemical pesticides
  • D. Plants being grown over several generations to produce larger fruit.
2. Bacteria are often used in genetic engineering. What feature makes them useful?
  • A. They have plasmids
  • B. Cell wall present
  • C. lack of a nucleus
  • D. Lack of a vacuole. 

Questions 3 to 9 are about using genetic engineering to manufacture human insulin.


insulin gene in chromosome


3. The gene for insulin is removed from a human chromosome using which enzyme?

  • A. ligase
  • B. Recombinase
  • C. Restriction enzyme
  • D. Hydrolase.

4.  The diagram is of a bacterium that might be used in genetic engineering:


Spaully | CC-BY-SA 2.5

Structures 1 and 2 are:

  Structure 1 Structure 2
A Bacterial DNA plasmid
B Chromosome Bacterial DNA
C Plasmid Chromosome
D Bacterial DNA Chromosome

5.The plasmids are removed from the bacterium and cut open. Why is the same restriction enzyme used to cut open the plasmid and to cut out the human insulin gene?

  • A. To save money
  • B. The laboratory only has one type of restriction enzyme
  • C. To save time
  • D. To provide complementary ‘sticky ends’

6+7: The image shows the insulin gene inserted into a plasmid.

subcloning partial diagram

Takometer | CC-BY-SA 2.5
6. The gene for insulin is inserted into the plasmid using .
7. In the recombinant plasmid what is found at A and B?
  • A. Glue
  • B. The enzyme ligase
  • C. Complementary base pairing of RNA of gene and plasmid
  • D. Complementary base pairing of DNA of gene and plasmid
8. For industrial production of insulin once the recombinant DNA has been inserted into bacterial cells, the bacteria are grown in ...
  • A. test tubes
  • B. industrial fermenters
  • C. petri dishes
  • D. conical flasks
9. Which of the following is not an advantage of using genetically engineered insulin to treat diabetes?
  • A. No ethical issues concerning the use of animals
  • B. No side effects that might be caused by animal insulin
  • C. No risk of transferring infections.
  • D. Some religions believe that man has no right to manipulate the course of nature.
10. Some species of crop plant have been engineered to produce their own insecticide that kills the natural pests of the crop plant. Why might this be a disadvantage to the farmer and the environment?
  • A. Sales of chemical insecticides are reduced.
  • B. Moth that lays its eggs in the crop plant dies
  • C. Biodiversity is reduced
  • D. Crop plant grows bigger